Search results for "Fungal Biochemistry"

showing 1 items of 1 documents

In Candida parapsilosis the ATC1 Gene Encodes for an Acid Trehalase Involved in Trehalose Hydrolysis, Stress Resistance and Virulence

2014

An ORF named CPAR2-208980 on contig 005809 was identified by screening a Candida parapsilosis genome data base. Its 67% identity with the acid trehalase sequence from C. albicans (ATC1) led us to designate it CpATC1. Homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two CpATC1 chromosomal alleles. Phenotypic characterization showed that atc1Δ null cells were unable to grow on exogenous trehalose as carbon source, and also displayed higher resistance to environmental challenges, such as saline exposure (1.2 M NaCl), heat shock (42°C) and both mild and severe oxidative stress (5 and 50 mM H2O2). Significant amounts of intracellular trehalose were …

Fungal PhysiologyMutantGlycobiologyTrehalase activitylcsh:MedicineMicrobiologiaPathogenesisPathology and Laboratory MedicineCandida parapsilosisBiochemistrychemistry.chemical_compoundNucleic AcidsMicrobial PhysiologyMedicine and Health SciencesTrehalaseTrehalaselcsh:ScienceFungal BiochemistryCandida albicansCandidaMultidisciplinaryVirulencebiologyOrganic CompoundsSalt ToleranceCatalaseEnzymesChemistryPhysical SciencesResearch ArticleGenes FungalMolecular Sequence DataCarbohydratesMycologyMicrobiologyMicrobiologyFungal ProteinsAmino Acid SequenceHeat shockGlycoproteinslcsh:ROrganismsFungiChemical CompoundsWild typeTrehaloseBiology and Life Sciencesbiology.organism_classificationTrehaloseYeastOxidative StressMetabolismchemistryProteolysisEnzymologylcsh:QHeat-Shock ResponsePLoS ONE
researchProduct